Includes bibliographical references and index.
|Statement||edited by Robert J. McMahon.|
|Series||Methods in molecular biology -- 418, Methods in molecular biology (Clifton, N.J.) -- v. 418.|
|Contributions||McMahon, Robert Joseph, 1958-|
|LC Classifications||QP552.A94 A95 2008|
|The Physical Object|
|Pagination||xiii, 232 p. :|
|Number of Pages||232|
|ISBN 10||1588295834, 1597455792|
|ISBN 10||9781588295835, 9781597455794|
|LC Control Number||2007933142|
Open Library is an open, editable library catalog, building towards a web page for every book ever published. Avidin-Biotin Interactions by Robert J. McMahon; 1 edition; First published in ; Subjects: Affinity chromatography, Protein Binding, Analytical Chemistry, Biotin, Avidin, Streptavidin, Affinity electrophoresis, Laboratory Manuals. ISBN: OCLC Number: Description: xiii, pages: illustrations ; 23 cm. Contents: Preparation of avidin conjugates / Rosaria P. Haugland and Mahesh K. Bhalgat --Coupling of antibodies with biotin / Rosaria P. Haugland and Wendy --Optimization of detection and quantification of proteins on . Avidin-Biotin Interactions by Robert J. McMahon, , available at Book Depository with free delivery : Robert J. Mcmahon. Free Online Library: Avidin-biotin interactions; methods and applications.(Brief Article, Book Review) by "SciTech Book News"; Publishing industry Library and information science Science and technology, general.
The interaction between avidin and biotin or streptavidin and biotin forms the basis of several widely used immunohistochemical techniques. An assumption inherent to these techniques is . Avidin-Biotin Interactions Autor Robert J. McMahon. This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. 1 The (Strept)Avidin–Biotin Interaction. Avidin is a glycoprotein found in egg-whites that contains four identical subunits of 16, Daltons each, giving an intact molecular weight of approximat (Green, ). Each subunit contains one binding site for biotin, or vitamin H, and one oligosaccharide modification (Asn-linked). Get this from a library! Avidin-biotin interactions: methods and applications. [Robert Joseph McMahon;] -- For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity.
Avidin-biotin interactions. Avidin-biotin interactions are also very commonly utilized for surface functionalization. Biotin is a small molecule and avidin is a tetrameric protein that has four binding sites, for which biotin has a high affinity. Biotin is able to bind to any of the four sites. Protein assemblies by site-specific avidin–biotin interactions Authors contributing to RSC publications (journal articles, books or book chapters) do not need to formally request permission to reproduce material contained in this article provided that the correct acknowledgement is given with the reproduced material. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology. The precise accumulation of protein functions on a nanoscale to fabricate advanced biomaterials has become possible by a bottom-up approach based on molecular avidin– biotin interaction is widely employed in the design of functional protein self-assemblies. Herein we assessed how the spatial arrangement of the avidin– biotin interaction between protein .